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Published on: September 16, 2022
In vitro evaluation of wound healing and antimicrobial potential of ozone therapy
Gabriel Álvares Borges1, Silvia Taveira Elias1, Sandra Márcia Mazutti da Silva1
1Faculty of Health Sciences, University of Brasília, Brasília, Brazil.
Abstract:
Although ozone therapy is extensively applied when wound repair and antimicrobial effect are necessary, little is known about cellular mechanisms regarding this process. Thus, this study aimed to evaluate ozone cytotoxicity in fibroblasts (L929) and keratinocytes (HaCaT) cell lines, its effects on cell migration and its antimicrobial activity. Cells were treated with ozonated phosphate-buffered saline (8, 4, 2, 1, 0.5 and 0.25 μg/mL ozone), chlorhexidine 0.2% or buffered-solution, and cell viability was determined through MTT assay. The effect of ozone on cell migration was evaluated through scratch wound healing and transwell migration assays. The minimum inhibitory concentrations for Candida albicans and Staphylococcus aureus were determined. Ozone showed no cytotoxicity for the cell lines, while chlorhexidine markedly reduced cell viability. Although no significant difference between control and ozone-treated cells was observed in the scratch assay, a considerable increase in fibroblasts migration was noticed on cells treated with 8 μg/mL ozonated solution. Ozone alone did not inhibit growth of microorganisms; however, its association with chlorhexidine resulted in antimicrobial activity. This study confirms the wound healing and antimicrobial potential of ozone therapy and presents the need for studies to elucidate the molecular mechanisms through which it exerts such biological effects.
Insights
Ozone therapy shows potential for wound healing and fighting microbes without harming cells. Further research is needed to understand the molecular mechanisms behind ozone
Area of Science:
- Biomedical science
- Cell biology
- Antimicrobial research
Background:
- Ozone therapy is widely used for wound repair and antimicrobial effects.
- Cellular mechanisms of ozone therapy remain largely unknown.
- Understanding ozone's biological effects is crucial for its clinical application.
Purpose of the Study:
- To investigate ozone cytotoxicity in fibroblast (L929) and keratinocyte (HaCaT) cell lines.
- To evaluate ozone's impact on cell migration.
- To determine ozone's antimicrobial activity against Candida albicans and Staphylococcus aureus.
Main Methods:
- Cells were exposed to varying concentrations of ozonated phosphate-buffered saline.
- Cell viability was assessed using the MTT assay.
- Cell migration was evaluated via scratch wound healing and transwell assays.
- Minimum inhibitory concentrations for microorganisms were determined.
Main Results:
- Ozone exhibited no cytotoxicity in L929 and HaCaT cell lines.
- Chlorhexidine significantly reduced cell viability.
- Ozone treatment, particularly at 8 μg/mL, enhanced fibroblast migration.
- Ozone alone did not inhibit microbial growth, but its combination with chlorhexidine demonstrated antimicrobial activity.
Conclusions:
- Ozone therapy demonstrates promising wound healing and antimicrobial potential.
- Ozone therapy is safe for fibroblasts and keratinocytes at tested concentrations.
- Further studies are required to elucidate the molecular mechanisms of ozone's biological effects.
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