Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictability of In-Office SureSmile<sup>®</sup> Clear Aligners: A Retrospective Analysis of Anterior Tooth Movements.

Dentistry journal·2026
Same author

Adipose-derived and bone marrow aspirate concentrate injections for osteoarthritis: A scoping review.

PM & R : the journal of injury, function, and rehabilitation·2026
Same author

Spiral phase infrared imaging with undetected photons using a visible wavelength spatial light modulator.

Scientific reports·2026
Same author

Human activity recognition at a kilometer range using single-photon LiDAR.

Optics express·2026
Same author

Developing a sexual assault emergency action plan: modified Delphi consensus recommendations from the American Medical Society for Sports Medicine.

British journal of sports medicine·2026
Same author

Doctors are at high risk of "complexity fatigue".

BMJ (Clinical research ed.)·2026
Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026
Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026
Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026
Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026
Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026
Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

Photorealistic Learned Landscapes for Augmented Reality
06:54

Photorealistic Learned Landscapes for Augmented Reality

Published on: June 27, 2025

837

Image reconstruction from photon sparse data.

Lena Mertens1, Matthias Sonnleitner1, Jonathan Leach2

  • 1School of Physics and Astronomy, University of Glasgow, Glasgow, G12 8QQ, UK.

Scientific Reports
|February 8, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a new algorithm for image reconstruction from photon-sparse data. The method improves image quality by minimizing deviations from measured data and incorporating regularization, leading to lower residuals.

More Related Videos

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.5K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.7K

Related Experiment Videos

Last Updated: Mar 7, 2026

Photorealistic Learned Landscapes for Augmented Reality
06:54

Photorealistic Learned Landscapes for Augmented Reality

Published on: June 27, 2025

837
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.5K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.7K

Area of Science:

  • Image processing
  • Computational imaging
  • Photonics

Background:

  • Image reconstruction often faces challenges with low photon counts, leading to noisy or inaccurate results.
  • Traditional methods struggle with photon-sparse data where photon counts per pixel are near unity.
  • Developing robust algorithms for low photon-number imaging is crucial for various scientific applications.

Purpose of the Study:

  • To develop and validate an algorithm for accurate image reconstruction from photon-sparse data.
  • To enhance image quality and reduce residuals compared to original measurements.
  • To demonstrate the algorithm's applicability across different photon-sparse data acquisition systems.

Main Methods:

  • An image optimization algorithm was developed, minimizing a cost function.
  • The cost function integrates a Poissonian log-likelihood term and a regularization term based on second spatial derivatives.
  • A bootstrapping technique was employed to balance the log-likelihood and regularization terms using smoothed data.

Main Results:

  • The developed algorithm successfully reconstructs images from photon-sparse data.
  • Processed images exhibited lower residuals when compared to the original data and the true object.
  • The technique was validated using data from both single-photon avalanche photodiode arrays and time-gated intensified cameras.

Conclusions:

  • The algorithm provides a significant improvement for image reconstruction with photon-sparse data.
  • The method demonstrates robustness and applicability across diverse low photon-number imaging systems.
  • This technique offers a valuable tool for enhancing image quality in low-light conditions.