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Related Experiment Videos

Molecular nature of the complement lesion.

S Bhakdi, J Tranum-Jensen

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1978
    PubMed
    Summary

    The terminal complement complex (C5b-9) can reinsert into lipid membranes, forming a transmembrane pore. This pore formation is crucial for complement-mediated cell damage.

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    Area of Science:

    • Immunology
    • Molecular Biology
    • Biochemistry

    Background:

    • The complement system is a key part of innate immunity.
    • Assembly of the terminal complement components (C5b-9) into a complex on target membranes causes cell damage.

    Purpose of the Study:

    • To investigate the structural and functional properties of the purified C5b-9 complex.
    • To determine if the C5b-9 complex can reincorporate into artificial lipid vesicles.

    Main Methods:

    • Isolation of C5b-9 complexes from target membranes.
    • Reconstitution of purified C5b-9 complexes into artificial lipid vesicles.
    • Analysis of the orientation and structure of the reincorporated complex.

    Main Results:

    • Purified C5b-9 complexes can be reincorporated into artificial lipid vesicles.
    • The C5b-9 complex adopts a vertically oriented, hollow, cylindrical structure.
    • The complex penetrates the lipid bilayer via lipid-binding regions, forming a transmembrane pore.

    Conclusions:

    • The C5b-9 complex possesses intrinsic properties allowing it to insert into lipid bilayers.
    • The formation of a transmembrane pore by the C5b-9 complex is a key mechanism of complement-mediated membrane attack.

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