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Related Experiment Video

Updated: Mar 7, 2026

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

BASIC: BCR assembly from single cells.

Stefan Canzar¹, Karlynn E Neu², Qingming Tang¹

¹Toyota Technological Institute at Chicago, Chicago, IL, USA

Bioinformatics (Oxford, England)

|February 8, 2017

Summary

This summary is machine-generated.

We developed BASIC, a new tool for assembling full-length B-cell receptor (BCR) sequences from single-cell RNA sequencing data. This method enables detailed analysis of BCRs at single-cell resolution.

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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

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Last Updated: Mar 7, 2026

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Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

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Published on: September 10, 2017

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Area of Science:

Immunology
Bioinformatics
Genomics

Background:

B-cell receptors (BCRs) are crucial for identifying diverse antigens.
Single-cell RNA sequencing (scRNA-seq) allows high-throughput transcript profiling.
Assembling full-length BCR heavy and light chain sequences from scRNA-seq data in B cells is challenging and underexplored.

Purpose of the Study:

To develop and validate a novel computational tool for assembling BCR sequences from scRNA-seq data.
To enable single-cell resolution analysis of BCR repertoire.

Main Methods:

Development of the BASIC software tool.
Application of scRNA-seq to nearly 200 single human B cells.
Experimental validation using single-cell primer-based nested PCRs and Sanger sequencing.

Main Results:

Successfully assembled full-length heavy and light chain BCR sequences for individual B cells.
Demonstrated the utility and accuracy of the BASIC tool through experimental validation.
Provided a method for high-throughput BCR sequence assembly at single-cell resolution.

Conclusions:

The BASIC tool facilitates the assembly of BCR sequences from scRNA-seq data.
This advancement enables deeper insights into B-cell populations and their antigen-recognition capabilities.
BASIC offers a valuable resource for immunological and genomic research.