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Isolation of cathepsin D using three-phase partitioning in t-butanol/water/ammonium sulfate
G R Jacobs1, R N Pike, C Dennison
1Department of Biochemistry, University of Natal, Pietermaritzburg, South Africa.
Analytical Biochemistry
|July 1, 1989
Abstract:
A 6-h procedure for the isolation of bovine cathepsin D is described. The procedure involves essentially only two steps; three-phase partitioning in t-butanol/water/ammonium sulfate followed by affinity chromatography on pepstatin-agarose. The major advantage of this new method over previous methods is the greatly reduced time required to obtain comparably pure cathepsin D.