Related Experiment Video
Updated: Aug 14, 2026

09:33
In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
Published on: January 5, 2016
An enzymatic assay for picomole levels of phosphatidate
S B Bocckino1, P Wilson, J H Exton
1Howard Hughes Medical Institute, Nashville, Tennessee.
Analytical Biochemistry
|July 1, 1989
Abstract:
An assay for phosphatidate that is sensitive, specific, and highly reproducible is described. Phosphatidate is transacylated with methylamine and the glycerol 3-phosphate produced is measured enzymatically. The assay is linear from 50 to 1500 pmol of phosphatidate and can be used to quantitate phosphatidate in small amounts of biological materials.

