Related Experiment Video

Updated: Mar 7, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

9.0K

microRNA-29c inhibits cell proliferation by targeting NASP in human gastric cancer

Beiqin Yu1, Xuehua Chen1, Jianfang Li1

  • 1Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No.197 Ruijin Er Road, Shanghai, 200025, People's Republic of China.

BMC Cancer
|February 9, 2017
PubMed
Abstract

Insights

MicroRNA-29c (miR-29c) is downregulated in gastric cancer, inhibiting cell proliferation and promoting apoptosis. This microRNA shows potential as an early biomarker and novel therapy target for this common malignancy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer is a prevalent malignancy globally.
  • MicroRNAs regulate cellular proliferation in gastric cancer.
  • MicroRNA-29c (miR-29c) functions as a tumor suppressor.

Purpose of the Study:

  • To investigate the role of miR-29c in gastric cancer.
  • To identify the molecular mechanisms underlying miR-29c's function.
  • To evaluate miR-29c as a potential diagnostic and therapeutic target.

Main Methods:

  • Quantitative PCR to assess miR-29c expression in patient tissues and cell lines.
  • Functional assays including proliferation, colony formation, apoptosis, and cell cycle analysis.
  • Bioinformatic prediction and experimental validation of miR-29c's target gene, Nuclear autoantigenic sperm protein (NASP).

Main Results:

  • miR-29c expression is significantly downregulated in gastric cancer tissues and cell lines.
  • Overexpression of miR-29c suppresses cell proliferation, induces apoptosis, and causes G1/G0 cell cycle arrest.
  • miR-29c targets NASP, and its depletion reverses the observed anti-cancer effects.

Conclusions:

  • miR-29c acts as a tumor suppressor in gastric cancer by inhibiting proliferation and promoting apoptosis.
  • miR-29c demonstrates potential as an early diagnostic biomarker.
  • miR-29c represents a promising novel therapeutic target for gastric cancer treatment.
Keywords:
Gastric cancerNASPProliferationmiR-29c

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K