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Things Are Getting Hairy: Enterobacteria Bacteriophage vB_PcaM_CBB.

Colin Buttimer¹, Hanne Hendrix², Hugo Oliveira³

¹Department of Biological Sciences, Cork Institute of Technology Cork, Ireland.

Frontiers in Microbiology

|February 9, 2017

Summary

Enterobacteria phage vB_PcaM_CBB is a jumbo phage with unique tail structures and a broad host range. Its large genome and protein similarities offer insights into phage diversity and jumbo phage biology.

Keywords:

Jumbo bacteriophage PFGE analysis bacteriophages bioinformatics genome host range mass spectrometry transmission electron microscopy

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Area of Science:

Microbiology
Virology
Genomics

Background:

Jumbo phages, members of the Myoviridae family, are large bacteriophages.
Enterobacteria phage vB_PcaM_CBB (phage CBB) is a newly characterized jumbo phage.
This phage exhibits unusual whisker-like structures on its contractile tail.

Purpose of the Study:

To present a detailed analysis of the atypical jumbo phage vB_PcaM_CBB.
To compare phage CBB with other known phages to understand its biological significance.
To contribute to the understanding of phage diversity and jumbo phage biology.

Main Methods:

Genome sequencing and analysis of phage CBB.
Proteome analysis of phage CBB virions.
Comparative analysis of phage CBB proteins with related phages.

Main Results:

Phage CBB has a large genome (355,922 bp) encoding 554 ORFs.
It possesses unique whisker-like structures on its contractile tail.
Phage CBB shares 33-38% of its proteins with related phages like Cronobacter phage GAP32 and Klebsiella phage vB_KleM_Rak2.
It infects species within the Erwinia, Pectobacterium, and Cronobacter genera.

Conclusions:

Phage CBB represents a highly atypical jumbo myoviridae phage.
Its unique characteristics expand the known diversity of bacteriophages.
This study enhances our understanding of jumbo phage biology and evolution.