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STAT3 and ERK pathways are involved in cell growth stimulation of the ST2/IL1RL1 promoter

Kenji Tago1, Satoshi Ohta1, Megumi Funakoshi-Tago2

  • 1Division of Structural Biochemistry Jichi Medical University Shimotsuke Tochigi Japan.

FEBS Open Bio
|February 9, 2017
PubMed

Insights

The ST2 gene

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Oncology

Background:

  • The ST2 gene is induced by growth factors and oncogenic stress.
  • ST2 possesses distinct distal and proximal promoters.

Purpose of the Study:

  • To identify novel regulatory elements in the ST2 proximal promoter.
  • To elucidate the role of STAT and ERK pathways in ST2 gene induction.

Main Methods:

  • Reporter gene analysis to identify serum-responsive elements.
  • Enforced expression of constitutively active STAT3 and oncogenic Ras (G12V).
  • Utilized pathway inhibitors (STAT3 and ERK).

Main Results:

  • A novel serum-responsive element in the ST2 proximal promoter was identified.
  • STAT3 activation and oncogenic Ras (G12V) induced ST2 gene expression via the proximal promoter.
  • STAT3 and ERK pathway inhibitors suppressed ST2 promoter activation.

Conclusions:

  • STAT family and ERK pathways are crucial for ST2 gene induction by growth stimulation and oncogenic Ras.
  • The ST2 proximal promoter plays a key role in mediating responses to growth factors and oncogenic stress.
Keywords:
ERK pathwayRasST2/IL1RL1STAT3promoter

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