Related Experiment Video
Updated: Mar 7, 2026
14:49
Associated Chromosome Trap for Identifying Long-range DNA Interactions
Published on: April 23, 2011
15.0K
Interaction trap experiment with CDC6.
Polona Bedina Zavec1, Aleksandra Comino1, Paul Watt2
1National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia;, , , , , , SI.
Pflugers Archiv : European Journal of Physiology
|February 9, 2017
Summary
Researchers identified proteins interacting with the essential yeast gene CDC6, revealing its potential roles in cell wall synthesis and carbon metabolism. Some interactions, like with Ty1 retrotransposition, also suppressed cdc6 mutations.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Cell Cycle Regulation
Background:
- The CDC6 gene is essential in Saccharomyces cerevisiae, but its protein's precise function in the cell cycle is not fully understood.
- Previous research has established the gene's DNA sequence but lacked detailed biochemical insights into Cdc6 protein activity.
Purpose of the Study:
- To identify proteins that specifically interact with the Cdc6 protein.
- To investigate the functional implications of these interactions for Cdc6's role in the cell cycle and cellular processes.
Main Methods:
- Employed the interaction trap experiment to screen for Cdc6-interacting proteins.
- Utilized sequence analysis to identify the nature and potential functions of the interacting partners.
- Measured interaction strength via β-galactosidase activity assays.
- Assessed the functional impact by observing the suppression of a cdc6-1 mutation.
Main Results:
- Identified four interacting partners: ECM11 (cell wall synthesis), YNL201 (potential carbon metabolism), YOR279 (unknown function), and the Ty1 retrotransposition element.
- Strongest interactions with Cdc6 were observed with ECM11 and YNL201, followed by YOR279, with the weakest interaction with Ty1A.
- Ty1A element showed the strongest suppression of the cdc6-1 mutation, with ECM11 and YNL201 providing slight suppression, while YOR279 showed no suppression.
Conclusions:
- Cdc6 protein interacts with diverse cellular components, including those involved in cell wall synthesis and potentially carbon metabolism.
- The Ty1 retrotransposition element's interaction with Cdc6 suggests a role in regulating or being regulated by the cell cycle.
- These findings provide new biochemical insights into the function of Cdc6 protein in yeast Saccharomyces cerevisiae.
