Related Experiment Video
Updated: Mar 7, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
Immobilization Increases the Stability and Reusability of Pigeon Pea NADP+ Linked Glucose-6-Phosphate Dehydrogenase
Siddhartha Singh1, Amit Kumar Singh2,3, M Chandrakumar Singh2
1Department of Basic Sciences & Humanities, College of Horticulture & Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, 791102, India. siddharthasingh786@gmail.com.
Abstract:
Immobilization of enzymes is valuably important as it improves the stability and hence increases the reusability of enzymes. The present investigation is an attempt for immobilization of purified glucose-6-phosphate dehydrogenase from pigeon pea on different matrix. Maximum immobilization was achieved when alginate was used as immobilization matrix. As compared to soluble enzyme the alginate immobilized enzyme exhibited enhanced optimum pH and temperature. The alginate immobilized enzyme displayed more than 80% activity up to 7 continuous reactions and more than 50% activity up to 11 continuous reactions.
More Related Videos
Related Concept Videos
Biosynthesis of Polysaccharides
Other Glycolytic Pathways
ATP Energy Storage and Release
One example of energy coupling using ATP involves a...
Glycolysis: Preparatory Phase
Glycolysis: Pay-off Phase
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

