Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single molecule super resolution microscopy reveals formation of Dengue NS2B3 cluster on mitochondrial network and its effect on fragmentation.

Npj viruses·2026
Same author

Unlocking Synergistic Ligand-Metal Interplay in Dual Redox-Active Metal-Organic Framework for High-Efficiency and Durable Overall Water Splitting.

ACS applied materials & interfaces·2026
Same author

Planar lightsheet optical tweezer pLOT for 2D trapping and imaging of freely-moving live cells.

Communications biology·2025
Same author

Role of the Chaperone Protein 14-3-3η in Regulation of the Infection Dynamics of the Influenza A (H1N1) Virus.

Viruses·2025
Same author

Gallium and germanium leaching from jarosite cake by organic acid: a combined experimental and DFT approach.

Journal of environmental management·2025
Same author

Photosensitized Energy-Transfer-Mediated Aminoformylation of Alkenes.

Organic letters·2025
Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026
Same journal

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Microscopy research and technique·2026
Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026
Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026
Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026
Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
10:18

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

Published on: January 27, 2017

15.2K

Light-sheet based lithography technique for patterning an array of microfluidic channels.

Kavya Mohan1, Partha Pratim Mondal1

  • 1Nanobioimaging Laboratory, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.

Microscopy Research and Technique
|February 9, 2017
PubMed
Summary
This summary is machine-generated.

We developed a light-sheet laser interference lithography method to create periodic microfluidic channels. This fast, single-shot technique fabricates 11.25-micron wide channels with precise periodicity using patterned light sheets.

Keywords:
fluorescencelightsheet microscopylithography

More Related Videos

One-Step Approach to Fabricating Polydimethylsiloxane Microfluidic Channels of Different Geometric Sections by Sequential Wet Etching Processes
08:31

One-Step Approach to Fabricating Polydimethylsiloxane Microfluidic Channels of Different Geometric Sections by Sequential Wet Etching Processes

Published on: September 13, 2018

10.5K
Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics
06:03

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

897

Related Experiment Videos

Last Updated: Mar 7, 2026

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
10:18

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

Published on: January 27, 2017

15.2K
One-Step Approach to Fabricating Polydimethylsiloxane Microfluidic Channels of Different Geometric Sections by Sequential Wet Etching Processes
08:31

One-Step Approach to Fabricating Polydimethylsiloxane Microfluidic Channels of Different Geometric Sections by Sequential Wet Etching Processes

Published on: September 13, 2018

10.5K
Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics
06:03

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

897

Area of Science:

  • Microfluidics
  • Optical Lithography
  • Materials Science

Background:

  • Fabricating periodic microfluidic channels is crucial for various applications.
  • Existing methods can be time-consuming or lack precision.
  • Light-sheet illumination offers potential for rapid, high-resolution patterning.

Discussion:

  • The proposed technique utilizes a spatial filter and cylindrical lens to generate a periodic light-sheet pattern.
  • This pattern is projected onto a photopolymer-coated glass substrate.
  • The 1D focusing property of the cylindrical lens creates line-shaped channel geometries.

Key Insights:

  • A novel light-sheet laser interference lithography technique is presented.
  • The method achieves a periodicity and width of approximately 11.25 microns for 1D periodic channels.
  • The process is a fast, single-shot fabrication method.

Outlook:

  • This technique can be optimized for fabricating complex microfluidic devices.
  • Potential applications include lab-on-a-chip systems and micro-reactors.
  • Further research could explore multi-layer or 3D microchannel fabrication.