Related Experiment Video
Updated: Mar 7, 2026

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
Published on: July 6, 2021
Resolving Late-Stage Intermediates of Eukaryotic Ribosome Assembly
Erik W Hartwick1, Brian T Wimberly1
1Department of Biochemistry and Molecular Genetics, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA.
Abstract:
The assembly of eukaryotic ribosomes requires about 200 assembly factors promoting RNA modification, folding, cleavage, and ribosomal protein association. In this issue of Structure, Johnson et al. (2017) report structures of several late-stage intermediates of pre-40S ribosomal subunit assembly. This work provides detailed testable insights into assembly factor function.
Related Concept Videos
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis
Improving Translational Accuracy
Ribosomes
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
Ribosomes
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
Directing Proteins to the Rough Endoplasmic Reticulum

