Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The α-(1,3)-glucan synthase gene agsE impacts the secretome of Aspergillus niger.

Antonie van Leeuwenhoek·2023
Same author

Heterogeneity in Spore Aggregation and Germination Results in Different Sized, Cooperative Microcolonies in an Aspergillus niger Culture.

mBio·2023
Same author

Quantifying Positional Isomers (QPI) by Top-Down Mass Spectrometry.

Molecular & cellular proteomics : MCP·2021
Same author

Feedback-Driven Assembly of the Axon Initial Segment.

Neuron·2019
Same author

MAP7 family proteins regulate kinesin-1 recruitment and activation.

The Journal of cell biology·2019
Same author

Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca<sup>2+</sup>/CaM Controls DCV Binding and Liprin-α/TANC2 Recruits DCVs to Postsynaptic Sites.

Cell reports·2018

Related Experiment Video

Updated: Mar 7, 2026

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

2.2K

Quantitative Map of Proteome Dynamics during Neuronal Differentiation.

Christian K Frese1, Marina Mikhaylova2, Riccardo Stucchi3

  • 1Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 Utrecht, the Netherlands; Netherlands Proteomics Centre, Padualaan 8, 3584 Utrecht, the Netherlands.

Cell Reports
|February 9, 2017
PubMed
Summary

This study maps protein changes during neuronal differentiation in rat hippocampal neurons. It reveals extensive proteome remodeling and identifies Neural Cell Adhesion Molecule 1 (NCAM1) as a key regulator of dendritic development.

Keywords:
NCAM1adhesion moleculedendrite outgrowthmass spectrometryneuronal developmentneuronal differentiationneuroproteomicsproteomequantitative proteomicsstable isotope labeling

More Related Videos

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation
06:50

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Published on: August 28, 2019

10.2K
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
06:24

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Published on: October 7, 2021

4.0K

Related Experiment Videos

Last Updated: Mar 7, 2026

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

2.2K
Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation
06:50

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Published on: August 28, 2019

10.2K
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
06:24

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Published on: October 7, 2021

4.0K

Area of Science:

  • Neuroscience
  • Proteomics
  • Cell Biology

Background:

  • Neuronal differentiation involves axon outgrowth, dendritogenesis, and synapse formation.
  • Understanding proteome dynamics is crucial for deciphering neurodevelopmental processes.

Purpose of the Study:

  • To systematically profile proteome dynamics throughout neuronal differentiation.
  • To create a quantitative map of neuronal proteome changes.

Main Methods:

  • Cultured rat hippocampal neurons at different developmental stages were used.
  • Stable isotope labeling and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) were employed.
  • Quantitative analysis of protein abundance changes was performed.

Main Results:

  • Nearly one-third of 4,500 quantified proteins showed a >2-fold expression change.
  • Extensive remodeling of the neuronal proteome was observed.
  • Neural Cell Adhesion Molecule 1 (NCAM1) was identified to promote dendritic arbor development via actin filament growth.

Conclusions:

  • The study provides a comprehensive quantitative map of neuronal proteome dynamics.
  • This resource facilitates further research into neurodevelopmental proteins.
  • NCAM1 plays a significant role in dendritic growth cone dynamics.