Restriction maps of the regions coding for methicillin and tobramycin resistances on chromosomal DNA in methicillin-resistant staphylococci
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K Ubukata1, R Nonoguchi, M Matsuhashi
1Department of Clinical Pathology, Teikyo University School of Medicine, Tokyo, Japan.
Antimicrobial Agents and Chemotherapy
|September 1, 1989
Abstract:
Chromosomal BamHI DNA fragments containing both the mecA gene encoding the penicillin-binding protein responsible for methicillin resistance and the aadD gene encoding 4',4"-adenylyltransferase responsible for tobramycin resistance were cloned from three methicillin- and tobramycin-resistant strains of Staphylococcus aureus and one strain of Staphylococcus epidermidis. Physical maps of the fragments were similar, suggesting their unique origin.