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Updated: Mar 7, 2026
Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
Published on: August 21, 2019
Renieramycin M Attenuates Cancer Stem Cell-like Phenotypes in H460 Lung Cancer Cells
Natchanun Sirimangkalakitti1, Supakarn Chamni1, Khanit Suwanborirux1
1Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi, Department of Pharmacognosy and Pharmaceutical Botany, Chulalongkorn University, Pathumwan, Bangkok, Thailand.
Background/Aim:
Cancer stem cells (CSCs) are a subpopulation of cancer cells that possess self-renewal and differentiation capacities. CSCs contribute to drug-resistance, cancer recurrence and metastasis, thus development of CSC-targeted therapeutic strategies has recently received significant attention in cancer research. In this study, the potential efficacy of renieramycin M (RM) isolated from the sponge Xestospongia species, was examined against lung CSCs.
Materials And Methods:
Colony and spheroid formation assays, as well as western blotting analysis of lung CSC protein markers were employed to determine the CSC-like phenotypes of H460 lung cancer cells after treatment with RM at non-toxic concentrations.
Results:
RM treatment reduced significantly colony and spheroid formation of H460 cells. Moreover, the CSC markers CD133, CD44 and ALDH1A1 of CSC-enriched H460 cells were reduced significantly following RM treatment.
Conclusion:
RM could be a potent anti-metastatic agent by suppressing lung CSC-like phenotypes in H460 cells.
Insights
Renieramycin M (RM) effectively suppressed lung cancer stem cell (CSC) characteristics in H460 cells. This marine compound reduced colony formation and key CSC markers, suggesting potential as an anti-metastatic therapy.
Area of Science:
- Marine natural products
- Cancer biology
- Drug discovery
Background:
- Cancer stem cells (CSCs) drive tumor growth, metastasis, and drug resistance.
- Targeting CSCs is crucial for effective cancer therapy.
- Renieramycin M (RM) is a compound isolated from the Xestospongia sponge.
Purpose of the Study:
- To investigate the efficacy of renieramycin M (RM) against lung cancer stem cells (CSCs).
- To evaluate RM's potential as a therapeutic agent for lung cancer.
Main Methods:
- H460 lung cancer cells were treated with non-toxic concentrations of RM.
- Colony and spheroid formation assays were performed.
- Western blotting was used to analyze CSC marker expression (CD133, CD44, ALDH1A1).
Main Results:
- RM significantly reduced colony and spheroid formation in H460 cells.
- RM treatment led to a significant decrease in CSC markers CD133, CD44, and ALDH1A1.
- These findings indicate RM suppresses CSC-like phenotypes.
Conclusions:
- Renieramycin M demonstrates potent activity against lung CSCs.
- RM may serve as a promising anti-metastatic agent by targeting CSCs.
- Further research into RM for lung cancer treatment is warranted.
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