Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of the Lymphatic System01:15

Development of the Lymphatic System

2.4K
The development of lymphatic tissues and vessels in embryonic life begins around the fifth week. These structures originate from the mesoderm layer, with lymph sacs emerging from developing veins.
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
2.4K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
Lymphatic Vessels and Lymph Transport01:16

Lymphatic Vessels and Lymph Transport

24.0K
Lymphatic vessels, known as lymphatics, are crucial in transporting lymph from peripheral tissues to our venous system. This process begins with lymph entering through tiny capillaries that branch through tissues. These capillaries have unique features such as larger diameters, thinner walls, and a distinctive one-way valve system formed by overlapping endothelial cells.
This one-way system allows fluids, solutes, and even pathogens to enter but prevents their return to the intercellular...
24.0K
Development of Blood Vessels01:07

Development of Blood Vessels

1.6K
The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
1.6K
Pleiotropy01:33

Pleiotropy

43.7K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
43.7K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.9K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hereditary spastic paraplegia (HSP) gene 11 (Spg11) attenuates lipid accumulation in myeloid cells and neuroinflammation in the midbrain without affecting α-synuclein pathology.

Journal of neuroinflammation·2026
Same author

Complement C3aR deletion does not attenuate degeneration in a tauopathy model or alter acute inflammation-induced gene expression changes.

Cell reports·2026
Same author

Corrigendum to "Laminin-511-functionalized fibrin gel enables in-gel proliferation of human induced pluripotent stem cells" [Matrix Biology Volume 142, December 2025, Pages 21-32].

Matrix biology : journal of the International Society for Matrix Biology·2026
Same author

P-LM421E8, the heparan sulfate chain-conjugated laminin-421-E8 fragment, drives differentiation of human induced pluripotent stem cells into hematopoietic progenitor cells comparable to basic fibroblast growth factor in a chemically defined system.

Matrix biology plus·2026
Same author

Covalently Platinated DNA Oligonucleotides as Ratiometric Dioxygen Sensors.

Bioconjugate chemistry·2025
Same author

Microthrombi growth in ADAMTS13 deficiency exacerbates inflammatory bowel disease via mucosal and endothelial dysfunction.

Journal of thrombosis and haemostasis : JTH·2025

Related Experiment Video

Updated: Mar 7, 2026

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

15.0K

An Evolutionarily Conserved Role for Polydom/Svep1 During Lymphatic Vessel Formation.

Terhi Karpanen1, Yvonne Padberg2, Serge A van de Pavert2

  • 1From the Hubrecht Institute, KNAW and UMC Utrecht, Utrecht, the Netherlands (T.K., S.A.v.d.P., J.P.-M., G.v.d.H., M.A., S.S.-M.); Institute of Cardiovascular Organogenesis and Regeneration, Faculty of Medicine, WWU Münster, Germany (Y.P., D.S., S.S.-M.); CiM Cluster of Excellence (EXC 1003-CiM), Münster, Germany (Y.P., D.S., S.S.-M.); Max Planck Institute for Molecular Biomedicine, Münster, Germany (C.D., F.K.); Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Suita, Japan (N.M., K.S.); and Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan (N.M.). schultes@ukmuenster.de Terhi.Karpanen@rr-research.no.

Circulation Research
|February 10, 2017
PubMed
Summary

The secreted Polydom/Svep1 protein is crucial for lymphatic vessel development. Studies in mice and zebrafish reveal its essential role in lymphangiogenesis, impacting sprouting, migration, and remodeling.

Keywords:
Polydom/Svep1arterieslymphangiogenesislymphatic vesselsmiceveinszebrafish

More Related Videos

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

18.2K
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

7.1K

Related Experiment Videos

Last Updated: Mar 7, 2026

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

15.0K
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

18.2K
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

7.1K

Area of Science:

  • Developmental Biology
  • Vascular Biology
  • Genetics

Background:

  • Lymphatic vessel formation and function are vital but genetically complex.
  • Understanding the genetic control of lymphangiogenesis is limited.

Purpose of the Study:

  • Identify the role of the secreted Polydom/Svep1 protein in lymphatic vasculature formation.
  • Analyze Polydom/Svep1 function in lymphangiogenesis using mouse and zebrafish models.

Main Methods:

  • Phenotypic analysis of zebrafish and mouse mutants for Polydom/Svep1.
  • Examination of lymphatic endothelial cell migration and vessel sprouting.
  • Analysis of gene expression patterns.

Main Results:

  • Zebrafish mutants showed reduced lymphovenous sprouting and impaired lymphatic cell migration.
  • Mouse mutants displayed abnormal lymphovenous contact site association and failed lymphatic remodeling.
  • Gene expression suggests a non-endothelial, non-cell autonomous mechanism.

Conclusions:

  • Polydom/Svep1 is an essential extracellular factor for lymphangiogenesis in both zebrafish and mice.
  • Mesenchymal cell expression of Polydom/Svep1 is required for lymphatic sprouting, migration, and remodeling.