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[A fluorimetric method for the determination of brain aminopeptidases]
Abstract:
A fluorometric method adapted to the determination of brain aminopeptidase activities has been developed in our laboratory. This method is based in the fluorescence produced by 2-naphthylamine when it is released from aminoacyl-2-naphthylamides under the hydrolytic action of these enzymes. Values of enzymatic activities and kinetic constants are similar to those previously described in the literature.