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Related Experiment Video

Updated: Mar 7, 2026

In Vivo Modeling of the Morbid Human Genome using Danio rerio

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Published on: August 24, 2013

Zebrafish atoh8 mutants do not recapitulate morpholino phenotypes.

Elsie S Place¹, James C Smith¹

¹Developmental Biology Laboratory, Francis Crick Institute, London, United Kingdom.

|February 10, 2017

Summary

Atoh8 is a bHLH transcription factor. Zebrafish homozygous for the atoh8sa1465 loss-of-function allele show normal development and survival, indicating Atoh8 is dispensable for zebrafish embryogenesis.

Area of Science:

Developmental biology
Genetics
Molecular biology

Background:

Atoh8 is a bHLH transcription factor involved in cell differentiation.
Previous studies on Atoh8 loss-of-function in mice and zebrafish yielded conflicting results.
The precise role of Atoh8 in vertebrate development remains unclear.

Purpose of the Study:

To genetically characterize zebrafish embryos homozygous for the atoh8sa1465 loss-of-function allele.
To provide genetic evidence for the developmental role of Atoh8 in zebrafish.
To clarify the phenotypic consequences of Atoh8 loss in zebrafish.

Main Methods:

Generated zebrafish embryos homozygous for the atoh8sa1465 loss-of-function allele.
Assessed embryonic morphology, swimbladder inflation, heart looping, and survival to adulthood.

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Last Updated: Mar 7, 2026

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Evaluated pericardial edema, sensitivity to Fog1 loss, vascular patterning, and primitive hematopoiesis.

Main Results:

Homozygous atoh8sa1465 zebrafish embryos exhibited normal body morphology, swimbladder inflation, and heart looping.
These embryos survived to adulthood without pericardial edema or sensitization to Fog1 loss.
Vascular patterning and primitive hematopoiesis were unaffected in atoh8sa1465/sa1465 mutant embryos.

Conclusions:

Atoh8 is dispensable for normal zebrafish development under standard laboratory conditions.
The previously described pericardial edema phenotype is not a specific consequence of Atoh8 loss.
This study provides genetic evidence clarifying the role of Atoh8 in zebrafish development.