[Methodologic variants of DNA blood stain analysis: extraction in agarose and digoxigenin marking]

DNA was extracted from 1 microliter bloodstains on glass, cotton cloth and paper using proteinase K digestion in a solution containing low gelling temperature agarose. This method is advantageous for small amounts of source material because the purifying procedures of other methods that contain a risk of loss of DNA, are avoided. The recovered DNA was digested with Hae III and after Southern blotting, a Digoxigenin labeled probe (Amprobe, human Y-chromosome specific repeat) was successfully used for sex determination. With this non-radioactive method quantities of DNA smaller than 1.5 pg were detected.