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Circular RNA and gene expression profiles in gastric cancer based on microarray chip technology
Weiguo Sui1, Zhoufang Shi2, Wen Xue1
1Nephrology Department of Guilin No. 181 Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541004, P.R. China.
Oncology Reports
|February 11, 2017
Summary
This study identified numerous differences in circular RNA (circRNA) and messenger RNA (mRNA) expression in gastric cancer (GC) tissues. These findings suggest circRNAs may serve as novel biomarkers for GC diagnosis and treatment.
Area of Science:
- Genomics
- Molecular Biology
- Cancer Research
Background:
- Gastric cancer (GC) is a significant global health concern with complex molecular underpinnings.
- Understanding differential gene and circular RNA (circRNA) expression is crucial for identifying novel diagnostic and therapeutic targets.
Purpose of the Study:
- To screen and analyze differences in messenger RNA (mRNA) and circRNA expression profiles in gastric cancer (GC) tissues compared to adjacent non-cancerous tissues.
- To investigate the potential mechanisms of circRNA involvement in gastric carcinoma, including interactions with microRNAs (miRNAs).
Main Methods:
- Agilent microarray technology was employed to generate differential expression profiles for circRNAs and mRNAs.
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was utilized to validate the expression levels of selected circRNAs and mRNAs.
- Gene Ontology (GO) and pathway analyses were performed on differentially expressed genes to understand their biological functions and associated pathways.
Main Results:
- A total of 1,285 differentially expressed circRNAs were identified, with 594 downregulated and 691 upregulated, often through interactions with miRNAs. Three specific circRNAs (hsa_circRNA_400071, hsa_circRNA_000543, hsa_circRNA_001959) were validated via qRT-PCR.
- Analysis revealed 5,460 differentially expressed genes (2,390 upregulated, 3,070 downregulated) in GC tissues. GO and pathway analyses indicated their involvement in various biological processes, cellular components, molecular functions, and signaling pathways.
- Sixty-nine differentially expressed circRNAs were found to potentially act as miRNA sponges, regulating the expression of target mRNAs. Genes such as CD44, CXXC5, MYH9, and MALAT1 were implicated in GC development through circRNA-miRNA-mRNA regulatory networks.
Conclusions:
- Differentially expressed circRNAs possess miRNA binding sites and regulate target gene expression, indicating their potential as novel molecular biomarkers for gastric cancer.
- The identified differentially expressed genes are implicated in the pathogenesis of GC through diverse molecular mechanisms.
- Specific genes (CD44, CXXC5, MYH9, MALAT1) may play critical roles in GC progression via intricate circRNA-miRNA-mRNA interactions.
