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Updated: Jul 11, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications
Kathy Brumbaugh1, Wen-Chie Liao2, J P Houchins2
1Bio-Techne, Inc., 614 McKinley Place NE, Minneapolis, MN, 55413, USA. Kathy.brumbaugh@bio-techne.com.
Abstract:
Phosphate addition is a posttranslational modification of proteins, and this modification can affect the activity and other properties of intracellular proteins. Different animal species can be used to generate phosphosite-specific antibodies as either polyclonals or monoclonals, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is vital for their use in proteomics and profiling of disease.
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