Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.3K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Agentic artificial intelligence in inflammatory bowel disease: toward autonomous and adaptive care.

Crohn's & colitis 360·2026
Same author

Early risk stratification of late-onset sepsis in very preterm infants by intestinal microbiota profiling: a multicenter case-control validation study.

Gut microbes·2026
Same author

REPROGRAM: REsilience PROmotion with GeRoprotectors: AssessMent of biological effect: Rationale and protocol for a trial of biological effect.

PloS one·2026
Same author

The human gut microbiome across the life course.

FEBS letters·2026
Same author

RNF43 Mutations Are Associated With the Classical Molecular Subtype, Vigorous Antitumor Immune Responses, and Prolonged Survival in Pancreatic Adenocarcinoma.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2026
Same author

Linking Targeted Pancreatic Cancer Genes With Metabolic Disorders: A Cross-Species Translational Pathway.

Cancer medicine·2026

Related Experiment Video

Updated: Mar 7, 2026

Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches
09:47

Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches

Published on: December 15, 2023

2.0K

Integration of metabolomics, lipidomics and clinical data using a machine learning method.

Animesh Acharjee1,2, Zsuzsanna Ament1, James A West1

  • 1Medical Research Council, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, UK.

BMC Bioinformatics
|February 11, 2017
PubMed
Summary

Dietary treatment with a PPAR-pan agonist altered liver metabolism in rats. Lipidomics data proved most predictive of dose responses and linked lipids to inflammation and metabolic processes.

More Related Videos

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
08:51

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts

Published on: September 20, 2024

2.2K
Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.4K

Related Experiment Videos

Last Updated: Mar 7, 2026

Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches
09:47

Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches

Published on: December 15, 2023

2.0K
Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
08:51

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts

Published on: September 20, 2024

2.2K
Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.4K

Area of Science:

  • Biochemistry
  • Toxicology
  • Metabolomics

Background:

  • Obesity and metabolic syndrome (MetS) necessitate novel drug targets.
  • Nuclear hormone receptors (NRs), particularly peroxisome proliferator-activated receptors (PPARs), are key regulators of lipid and carbohydrate metabolism.
  • While PPAR agonists show therapeutic potential for MetS, long-term effects and pathological changes remain incompletely understood.

Purpose of the Study:

  • To investigate the long-term effects of a PPAR-pan agonist on liver metabolism in male Sprague Dawley rats.
  • To integrate classical toxicology with high-throughput metabolomics and lipidomics data.
  • To identify key metabolic and lipidomic changes associated with PPAR-pan agonist treatment.

Main Methods:

  • Rats were administered a PPAR-pan agonist (PPAR-α, -γ, -δ) via dietary treatment.
  • Liver tissue was analyzed using classical toxicology (clinical chemistry) and mass spectrometry-based metabolomics and lipidomics.
  • A data-driven machine learning approach was employed to integrate nine multivariate datasets.

Main Results:

  • Lipidomics data, specifically Direct Infusion-Mass Spectrometry, demonstrated the highest predictive power for dose responses.
  • Significant associations were found between metabolic/lipidomic data and liver enzymes aspartate amino transaminase (AST) and albumin.
  • Correlations and network analyses revealed lipids (eicosanoids, phospholipids, triacylglycerols) as crucial links between inflammation and intermediary metabolism.

Conclusions:

  • Lipidomics is a powerful tool for predicting dose-dependent responses to metabolic interventions.
  • PPAR-pan agonist treatment impacts liver function, as indicated by changes in AST and albumin levels.
  • Lipids play a central role in connecting inflammatory pathways with metabolic regulation in the liver.