Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hexose transport in human myoblasts.

O T Mesmer1, T C Lo

  • 1Department of Biochemistry, University of Western Ontario, London, Canada.

The Biochemical Journal
|August 15, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraluminal brachytherapy for malignant endobronchial tumors: an update on low-dose rate versus high-dose rate radiation therapy.

Clinical lung cancer·2003
Same author

Internet anatomy 101: knowing the turf you're going to surf.

Administrative radiology journal : AR·2002
Same author

Re-irradiation for prophylaxis of heterotopic ossification after hip surgery.

The British journal of radiology·2001
Same author

A single point mutation in the V3 region affects protein kinase Calpha targeting and accumulation at cell-cell contacts.

Molecular and cellular biology·2001
Same author

A case of isolated ACTH deficiency presenting with hypercalcaemia.

International journal of clinical practice·2001
Same author

Outcome of renal replacement therapy in the very elderly.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2001

Human myoblasts have high- (HAHT) and low- (LAHT) affinity hexose transport. Duchenne muscular dystrophy (DMD) myoblasts also exhibit a super-high-affinity hexose transport system (SHAHT), unique to DMD.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Human skeletal muscle cells utilize hexose transport systems for energy.
  • Understanding these systems is crucial for metabolic research and disease investigation.

Purpose of the Study:

  • To characterize hexose transport properties in normal human myoblasts.
  • To investigate alterations in hexose transport in Duchenne muscular dystrophy (DMD) myoblasts.

Main Methods:

  • Isolation of human myoblasts from normal subjects and DMD patients.
  • Analysis of hexose uptake kinetics using non-metabolizable analogues (e.g., 2-deoxyglucose, 3-O-methyl-D-glucose).
  • Assessment of transporter responses to inhibitors (cytochalasin B, phloretin, phlorizin) and energy uncouplers.

Related Experiment Videos

Main Results:

  • Normal human myoblasts exhibit high-affinity (HAHT) and low-affinity (LAHT) hexose transport systems.
  • DMD myoblasts possess HAHT and LAHT, plus a novel super-high-affinity hexose transport system (SHAHT).
  • SHAHT in DMD myoblasts shows distinct substrate affinity and differential responses to inhibitors and uncouplers compared to HAHT.

Conclusions:

  • Human skeletal muscle cells employ multiple hexose transport systems.
  • Duchenne muscular dystrophy is associated with a unique super-high-affinity hexose transporter not found in other myopathies.