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Disturbed mitochondrial function restricts glutamate uptake in the human Müller glia cell line, MIO-M1
Rupali Vohra1, Iswariyaraja Sridevi Gurubaran2, Ulrik Henriksen2
1University of Copenhagen, Department of Neuroscience and Pharmacology, Blegdamsvej 3B, Building 33.4.83, Copenhagen N, Denmark; University of Copenhagen, Department of Drug Design and Pharmacology, Universitetsparken 2, Building 22.4.405, 2100 Copenhagen Ø, Denmark; Zealand University Hospital, Department of Ophthalmology, Vestermarksvej 23, 4000 Roskilde, Denmark.
Abstract:
Using the human Müller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in Müller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were studied in response to mitochondrial inhibition and glucose restriction. Lactate release decreased in response to glucose restriction. Combined glucose restriction and blocked mitochondrial activity decreased survival and caused collapse of the respiratory chain measured by oxygen consumption rate and extracellular acidification rate. Mitochondrial inhibition caused impaired glutamate uptake and decreased mRNA expression of the glutamate transporter, EAAT1. Over all, we show important roles of mitochondrial activity in MIO-M1 cell function and survival.
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