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Related Experiment Videos

Enkephalin-binding protein in human blood.

S Nakajima1, T Komuro, M Shimamura

  • 1Department of Anesthesiology, School of Medicine, Juntendo University, Tokyo, Japan.

Biochemistry International
|September 1, 1989
PubMed
Summary
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Researchers identified a novel enkephalin-binding protein in human plasma. This protein

Area of Science:

  • Biochemistry
  • Neuroscience
  • Proteomics

Background:

  • Enkephalins are endogenous opioid peptides involved in pain modulation and other physiological processes.
  • The existence and function of specific enkephalin-binding proteins in circulation remain incompletely understood.

Purpose of the Study:

  • To identify and characterize a protein in human plasma that binds enkephalins.
  • To investigate the binding characteristics and potential physiological significance of this plasma protein.

Main Methods:

  • Partial purification of the enkephalin-binding protein from human plasma and serum using DEAE-cellulose column chromatography.
  • Competitive binding assays using radiolabeled leucine-enkephalin and various unlabeled peptides.

Main Results:

Related Experiment Videos

  • A protein capable of binding [3H]leucine-enkephalin was isolated from human plasma.
  • Binding was competitively inhibited by leucine-enkephalin, methionine-enkephalin, beta-endorphin, and glucagon.
  • Inhibition was not observed with Leu-enkephalin-amide, suggesting the C-terminus is crucial for binding.

Conclusions:

  • A novel enkephalin-binding protein exists in human plasma.
  • The C-terminal region of enkephalins plays a significant role in their interaction with this protein.
  • Further research is warranted to elucidate the physiological roles of this circulating enkephalin-binding protein.