Green synthesis of silver nanoparticle for the selective and sensitive colorimetric detection of mercury (II) ion

An ecofriendly and zero cost approach has been developed for the photoinduced synthesis of more stable AgNPs using an aqueous extract of Murraya koenigii (AEM) as a reducing and stabilizing agent. The exposed reaction mixture of AEM and AgNO₃ to sunlight turned dark brown which primarily confirmed the biosynthesis of AgNPs. The biosynthesis was monitored by UV-vis spectroscopy which exhibited a sharp SPR band at 430nm after 30min of sunlight exposure. The optimum conditions for biosynthesis of AgNPs were 30min of sunlight exposure, 2.0% (v/v) of AEM inoculuam dose and 4.0mM AgNO₃ concentration. TEM analysis confirmed the presence of spherical AgNPs with average size 8.6nm. The crystalline nature of the AgNPs was confirmed by XRD analysis where the Bragg's diffraction pattern at (111), (200), (220) and (311) corresponded to face centered cubic crystal lattice of metallic silver. The surface texture was analyzed by AFM analysis where the average roughness of the synthesized AgNPs was found 1.8nm. FTIR analysis was recorded between 4000 and 400cm^-1 which confirmed the involvement of various functional groups in the synthesis of AgNPs. On the basis of the linear relationship between SPR band intensity and different concentration of Hg²⁺, the synthesized AgNPs can be used for colorimetric detection of Hg²⁺ with a linear range from 50nm to 500μM. Based on experimental findings, an oxidation-reduction mechanism between AgNPs and Hg²⁺ was also proposed.