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Related Experiment Videos

Coordinated events of nuclear assembly.

Dollie LaJoie1, Katharine S Ullman1

  • 1Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA.

Current Opinion in Cell Biology
|February 12, 2017
PubMed
Summary

During open mitosis, the cell dismantles and rebuilds the nucleus. Nuclear envelope proteins and membrane recruit to chromatin, forming distinct regions that eventually coalesce to reform the nucleus.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Metazoan cells dismantle their nucleus during open mitosis to segregate chromosomes.
  • Reassembly of the nuclear envelope and architecture follows chromosome separation.

Purpose of the Study:

  • To investigate the dynamic process of nuclear envelope reassembly after mitosis.
  • To understand the role of chromatin and specific proteins in nuclear reformation.

Main Methods:

  • Observation of nuclear envelope formation in real-time.
  • Analysis of protein recruitment to chromatin.
  • Study of membrane dynamics and pore formation.

Main Results:

  • Nuclear envelope proteins are attracted to chromatin, initiating membrane recruitment.
  • Distinct regions with varying pore formation and microtubule interactions emerge.
  • Microtubule attachments act as barriers to nuclear envelope sealing.

Conclusions:

  • Nuclear envelope reassembly is a spatially and temporally regulated process.
  • Coalescence of distinct nuclear envelope regions leads to the formation of a functional nucleus.
  • Remodeling of microtubule-kinetochore interactions is crucial for nuclear sealing.

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