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Author Spotlight: Advancing Therapeutics with Biocompatible Sodium Alginate Hydrogel Microspheres
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An injectable alginate-based hydrogel for microfluidic applications.

Seref Akay1, Rene Heils2, Hoc Khiem Trieu3

  • 1Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova, Izmir, Turkey; Department of Genetic & Bioengineering, Faculty of Engineering, Gumushane University, 29100 Gumushane, Turkey.

Carbohydrate Polymers
|February 13, 2017
PubMed
Summary

This study presents an injectable alginate formulation for enzyme immobilization in microfluidic systems. The developed method ensures stable enzyme activity and complete substrate conversion over 24 hours.

Keywords:
AlginateChitosanHydrogelMicrofabricationMicrofluidicsMonolith

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Area of Science:

  • Biochemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Enzyme immobilization is crucial for biocatalysis in microfluidic devices.
  • Alginate hydrogels offer biocompatibility but require controlled gelation for enzyme encapsulation.
  • Developing stable and efficient enzyme delivery systems for microfluidics is an ongoing challenge.

Purpose of the Study:

  • To develop an injectable alginate-based formulation for enzyme immobilization within microfluidic systems.
  • To investigate the impact of d-glucono-δ-lactone (GDL) concentration on gelation time and enzymatic activity.
  • To enhance enzyme stability during immobilization using chitosan.

Main Methods:

  • An injectable alginate formulation was prepared, with gelation induced by GDL and calcium ions released from CaCO3.
  • The effect of GDL concentration on enzyme activity and gelation kinetics was evaluated.
  • Chitosan was incorporated at varying ratios to improve enzyme stability.
  • A microfluidic chip with a spiral coil channel was fabricated and filled with the enzyme-laden alginate mixture before gelation.

Main Results:

  • Increasing GDL concentration positively correlated with increased surface area and enzymatic activity.
  • Chitosan addition enhanced the stability of the immobilized enzyme.
  • Continuous substrate conversion was achieved for 24 hours within the microfluidic system.
  • No enzyme leakage or deactivation was observed during the continuous operation.

Conclusions:

  • The developed injectable alginate formulation provides an effective method for enzyme immobilization in microfluidic devices.
  • The formulation ensures high enzymatic activity, stability, and long-term operational performance.
  • This approach holds promise for advanced microfluidic applications in biocatalysis and diagnostics.