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Transcriptional and post-transcriptional regulation of Cdc20 during the spindle assembly checkpoint in S. cerevisiae

Ruiwen Wang1, Janet L Burton2, Mark J Solomon2

  • 1Institute of Life Sciences, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian Province 350108, China.

Cellular Signalling
|February 13, 2017
PubMed

Insights

The anaphase-promoting complex (APC), a key cell cycle regulator, degrades Cdc20 via cis-degradation during the spindle assembly checkpoint (SAC). This process, along with feedback regulation, ensures proper cell cycle arrest.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The anaphase-promoting complex (APC) is a crucial ubiquitin ligase regulating the cell cycle.
  • Cdc20 acts as an activator and substrate-binding protein for the APC.
  • Cdc20 degradation via APC in normal cycling cells primarily occurs through cis-degradation.

Purpose of the Study:

  • To elucidate the degradation mechanism of Cdc20 during the spindle assembly checkpoint (SAC).
  • To investigate the role of cis-degradation in Cdc20 turnover during SAC.
  • To explore the relationship between APCCdc20 activity and CDC20 gene transcription.

Main Methods:

  • Utilized a dual-Cdc20 system to study Cdc20 degradation.
  • Investigated the mechanism of Cdc20 degradation during the spindle assembly checkpoint.
  • Analyzed the transcriptional activity of the CDC20 promoter in relation to APCCdc20 activity.

Main Results:

  • Cis-degradation is identified as the major pathway for Cdc20 degradation during the spindle assembly checkpoint (SAC).
  • An inverse relationship was observed between APCCdc20 activity and CDC20 promoter transcriptional activity.
  • Feedback regulation by APCCdc20 substrates, including cyclins Clb2 and Clb5, likely mediates this inverse relationship.

Conclusions:

  • Cdc20 degradation during SAC predominantly occurs via cis-degradation.
  • Feedback regulation mechanisms involving APCCdc20 substrates modulate CDC20 transcription.
  • These findings highlight the importance of APCCdc20 inhibition and Cdc20 degradation for effective spindle checkpoint arrest.
Keywords:
Anaphase-promoting complexCdc20Post-transcriptional regulationProtein degradationSpindle assembly checkpointUbiquitination

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