Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Large field of view fluorescence imaging of microfluidic devices with a tandem-lens macroscope.

Lab on a chip·2026
Same author

High-throughput biochemical phenotyping of SHP2 variants reveals the molecular basis of diseases and allosteric drug inhibition.

bioRxiv : the preprint server for biology·2026
Same author

FoTO1 orchestrates Taxol biosynthesis through catalytic and non-catalytic mechanisms.

bioRxiv : the preprint server for biology·2026
Same author

Engineering the mechanosensitivity of single DNA molecules via high-throughput microfluidic force spectroscopy.

bioRxiv : the preprint server for biology·2026
Same author

uSort-M: Scalable isolation of user-defined sequences from diverse pooled libraries.

bioRxiv : the preprint server for biology·2026
Same author

Functional Characterization of Glucokinase Variants to Aid Clinical Interpretation of Monogenic Diabetes.

International journal of molecular sciences·2026
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
10:18

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

Published on: January 27, 2017

15.2K

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices.

Kara Brower1, Adam K White2, Polly M Fordyce3

  • 1Department of Bioengineering, Stanford University; Microfluidic Foundry, Stanford University; Chem-H Institute, Stanford University.

Journal of Visualized Experiments : Jove
|February 13, 2017
PubMed
Summary
This summary is machine-generated.

This study provides a detailed photolithography protocol for creating complex multilayer microfluidic devices. This empowers non-specialists to fabricate custom devices for biological applications.

More Related Videos

Design and Development of a Three-Dimensionally Printed Microscope Mask Alignment Adapter for the Fabrication of Multilayer Microfluidic Devices
06:21

Design and Development of a Three-Dimensionally Printed Microscope Mask Alignment Adapter for the Fabrication of Multilayer Microfluidic Devices

Published on: January 25, 2021

3.4K
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

4.6K

Related Experiment Videos

Last Updated: Mar 7, 2026

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
10:18

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

Published on: January 27, 2017

15.2K
Design and Development of a Three-Dimensionally Printed Microscope Mask Alignment Adapter for the Fabrication of Multilayer Microfluidic Devices
06:21

Design and Development of a Three-Dimensionally Printed Microscope Mask Alignment Adapter for the Fabrication of Multilayer Microfluidic Devices

Published on: January 25, 2021

3.4K
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

4.6K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Materials Science

Background:

  • Microfluidic systems offer advanced high-throughput analysis but have a steep learning curve for non-specialists.
  • Lack of detailed photolithography protocols hinders custom microfluidic device development, especially for complex features.

Purpose of the Study:

  • To provide a comprehensive, accessible protocol for fabricating multilayer microfluidic devices with complex geometries and integrated valves.
  • To enable non-specialists to design and create novel microfluidic devices for diverse research applications.

Main Methods:

  • Detailed photolithography procedures for multi-height patterning and feature rounding.
  • Fabrication of silicon masters for polydimethylsiloxane (PDMS) microfluidic device replication.
  • Demonstration using a microfluidic hydrogel bead synthesizer for droplet polymerization.

Main Results:

  • Successful fabrication of multilayer microfluidic devices with integrated valves and complex multi-height features.
  • Demonstrated production of polymerizable droplets for hydrogel bead synthesis.
  • Protocol provides a foundation for reproducible microfluidic device fabrication.

Conclusions:

  • The provided protocol demystifies photolithography for microfluidic device fabrication, lowering the barrier to entry for researchers.
  • Enables the development of custom microfluidic devices, accelerating innovation in biological laboratories.
  • Facilitates the application of advanced microfluidic technologies across a wider range of scientific disciplines.