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Related Experiment Video

Updated: Mar 7, 2026

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

Published on: February 3, 2017

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy.

Marco Morsch¹, Rowan A W Radford², Emily K Don²

¹Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University; marco.morsch@mq.edu.au.

Journal of Visualized Experiments : Jove

|February 13, 2017

Summary

A refined UV laser ablation method allows real-time visualization of cellular responses to injury in the central nervous system (CNS). This technique enables detailed study of single-cell fates and immediate interactions with surrounding cells like microglia and astrocytes.

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Last Updated: Mar 7, 2026

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

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Published on: February 3, 2017

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Area of Science:

Neuroscience
Cell Biology
Microscopy

Background:

Studying cell-specific responses to injury in the CNS is challenging due to complex setups and non-physiological environments.
Existing ablation methods like drug-mediated or high-power pulsed laser ablation lack specificity or require specialized equipment, hindering real-time visualization.

Purpose of the Study:

To develop and validate a refined UV laser ablation method for selective, real-time cellular injury induction and visualization in the CNS.
To overcome limitations of previous methods for studying single-cell responses and cell-cell interactions post-injury.

Main Methods:

Utilized a standard confocal microscope equipped with a 405-nm laser for UV ablation.
Applied the method to selectively ablate single neurons in the zebrafish spinal cord.
Visualized dose- and time-dependent cellular responses and degeneration in real-time.

Main Results:

Demonstrated selective ablation of individual neurons within dense cellular networks.
Observed dose-dependent neuronal fragmentation and anterograde axonal degeneration within minutes to hours.
Enabled real-time visualization of immediate responses from surrounding cells, including microglia and astrocytes.

Conclusions:

The refined UV laser ablation method provides a powerful, accessible tool for studying cellular injury and responses in the CNS.
This technique facilitates the investigation of individual cell fates and the dynamic interactions of glial cells with dying neurons in vivo.