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Published on: January 8, 2016
A Label-free "Turn-on" Fluorescence Method for Detecting Mercury Ion in Aqueous Solution Based on N-Methyl
Xingping Zhang1, Baomiao Ding, Huawei Wu
1Jingchu Food Research and Development Center, College of Life Science, Yangtze University.
Abstract:
In the present work, a simple design of "turn-on" fluorescence method for mercury ion was developed and explored based on specific T-Hg-T mismatches as a recognizer and N-methyl mesoporphyrin IX (NMM)/G-quadruplex DNA system as a reporter. The titration experiment showed that the mercury-ion concentration and the fluorescence intensity signal change exhibited a consistent linear correlation within the 50 to 500 nM range with a detection limit down to 12.9 nM. In a selectivity experiment, our method showed obvious selectivity against other metal ions, being consistent with other reported detection methods of mercury ion based on the specific reactivity of T-Hg-T mismatches. Our method was then successfully employed to detect mercury ion in pond water with excellent reliability.
