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Databases for technical aspects of immunohistochemistry

Satoshi Furukawa1, Mika Nagaike2, Kiyokazu Ozaki3

  • 1Conference on Experimental Animal Histopathology; Biological Research Laboratories, Nissan Chemical Industries, Ltd., 1470 Shiraoka, Shiraoka-shi, Saitama 349-0294, Japan.

Journal of Toxicologic Pathology
|February 14, 2017
PubMed

With the aims of sharing information about the technical aspects of immunohistochemistry (IHC) and making it possible to make a suitable choice of antibody for histopathological examination, this technical report describes the results of a questionnaire administered during the period of 2014 to 2015 to members of the Conference on Experimental Animal Histopathology. It also describes the immunological properties of primary antibodies (clone, supplier, catalog number, species reactivity, etc.) and the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, secondary antibody information, etc.) for a total number of 733 primary antibodies (425 kinds of primary antibody).

Keywords:
antibodyimmunohistochemistrytoxicological pathology

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