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Immunocytochemistry and Immunohistochemistry

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Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Updated: Mar 7, 2026

Histological-Based Stainings Using Free-Floating Tissue Sections

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

Databases for technical aspects of immunohistochemistry.

Satoshi Furukawa¹, Mika Nagaike², Kiyokazu Ozaki³

¹Conference on Experimental Animal Histopathology; Biological Research Laboratories, Nissan Chemical Industries, Ltd., 1470 Shiraoka, Shiraoka-shi, Saitama 349-0294, Japan.

Journal of Toxicologic Pathology

|February 14, 2017

Summary

This summary is machine-generated.

This report details technical aspects of immunohistochemistry (IHC) and antibody selection for histopathology. It provides data on 733 primary antibodies and their staining conditions, aiding researchers in choosing optimal reagents.

Keywords:

antibody immunohistochemistry toxicological pathology

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Last Updated: Mar 7, 2026

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Area of Science:

Histopathology
Immunohistochemistry
Experimental Animal Research

Background:

Immunohistochemistry (IHC) is crucial for histopathological examination.
Selecting appropriate antibodies and optimizing staining conditions are critical for reliable results.
Technical variability can impact IHC outcomes.

Purpose of the Study:

To share technical information regarding immunohistochemistry (IHC).
To facilitate informed antibody selection for histopathological examinations.
To document immunological properties and staining conditions for a large set of primary antibodies.

Main Methods:

A questionnaire was administered to members of the Conference on Experimental Animal Histopathology (2014-2015).
Data collected included immunological properties of primary antibodies (clone, supplier, catalog number, species reactivity).
IHC staining conditions were detailed (fixing, antigen retrieval, antibody dilution, incubation, controls, etc.).

Main Results:

The report describes 733 primary antibodies, representing 425 distinct antibody types.
Detailed information on antibody properties and specific IHC staining protocols is provided.
This comprehensive dataset serves as a valuable resource for IHC users.

Conclusions:

Standardizing IHC protocols and antibody characterization is essential.
This technical report provides a practical guide for optimizing IHC experiments.
The data aids in selecting suitable antibodies and conditions for accurate histopathological diagnosis.