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Related Experiment Video

Updated: Mar 7, 2026

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Published on: April 15, 2017

Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration.

Zhizeng Gao¹, Masahiro Niikura², Stephen G Withers¹

¹Department of Chemistry, University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada.

Angewandte Chemie (International Ed. in English)

|February 14, 2017

Summary

Researchers developed a new reagent to accurately measure active influenza neuraminidase in samples. This tool aids in developing better anti-influenza drugs and analyzing influenza vaccines.

Keywords:

active-site titration enzymes influenza neuraminidase vaccines

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Last Updated: Mar 7, 2026

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Area of Science:

Virology
Biochemistry
Drug Development

Background:

Influenza viral neuraminidase is critical for infection and a target for antiviral drugs and vaccines.
Current methods lack accuracy and efficiency for measuring active neuraminidase in complex samples, hindering research and development.

Purpose of the Study:

To develop a selective and sensitive reagent for quantifying active viral neuraminidase concentrations.
To enable accurate measurement of neuraminidase activity in crude samples, including virus preparations and vaccines.

Main Methods:

Development of a novel active-site titration reagent specific for viral neuraminidase.
Application of the reagent to quantify sub-nanomolar levels of neuraminidase in crude mixtures.
Determination of kinetic parameters (kcat) for multiple influenza A and B neuraminidases.

Main Results:

The developed reagent accurately quantifies viral neuraminidase down to sub-nanomolar levels in crude samples.
The reagent shows no background interference from non-viral neuraminidases.
Accurate kcat values were determined for eight different influenza neuraminidases.
Neuraminidase content in a commercial influenza vaccine was successfully quantified.

Conclusions:

The new titration reagent provides a sensitive and selective method for measuring active viral neuraminidase.
This tool facilitates the development of novel anti-influenza therapeutics and improves vaccine analysis.
Enables precise characterization of viral neuraminidases and their quantification in vaccine formulations.