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The Ndc80 complex bridges two Dam1 complex rings.

Jae Ook Kim1, Alex Zelter1, Neil T Umbreit1

  • 1Department of Biochemistry, University of Washington, Seattle, United States.

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Summary
This summary is machine-generated.

The Ndc80 complex binds two Dam1 complex rings at the Saccharomyces cerevisiae kinetochore, a crucial interaction for cell division. Aurora B kinase regulates this binding, and incorrect spacing disrupts chromosome segregation.

Keywords:
Dam1Ndc80S. cerevisiaebiochemistrycell biologykinetochoremicrotubulemitosis

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Kinetochore-microtubule attachments are vital for accurate chromosome segregation during mitosis.
  • The Dam1 and Ndc80 complexes are key microtubule-binding components of the Saccharomyces cerevisiae kinetochore.
  • Aurora B kinase regulates the interaction between these complexes to enhance kinetochore-microtubule coupling.

Purpose of the Study:

  • To investigate the tripartite interaction between the Ndc80 and Dam1 complexes.
  • To determine the role of Aurora B kinase in regulating this interaction.
  • To elucidate the in vivo significance of the Ndc80-Dam1 complex interaction for kinetochore function.

Main Methods:

  • In vitro binding assays to assess Ndc80 complex's ability to bind Dam1 rings.
  • Site-directed mutagenesis of Ndc80p interaction regions.
  • In vivo studies using Saccharomyces cerevisiae to evaluate kinetochore-microtubule attachment and chromosome segregation.

Main Results:

  • The Ndc80 complex can simultaneously bind two Dam1 complex rings via a tripartite interaction.
  • Aurora B kinase regulates each component of this tripartite interaction.
  • Mutations disrupting the interaction abrogate binding of two Dam1 rings in vitro and cause defects in kinetochore biorientation and microtubule attachment in vivo.
  • An engineered extra-long Ndc80 complex, spacing Dam1 rings further apart, failed to support growth.

Conclusions:

  • Each kinetochore in vivo contains two Dam1 rings.
  • The proper spacing between these Dam1 rings, mediated by the Ndc80 complex, is essential for kinetochore-microtubule attachment and faithful chromosome segregation.
  • Aurora B kinase plays a critical regulatory role in this process.