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Updated: Mar 7, 2026

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
Published on: December 16, 2022
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes
Brett T Staahl1, Madhurima Benekareddy2, Claire Coulon-Bainier2
1Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.
Abstract:
We demonstrate editing of post-mitotic neurons in the adult mouse brain following injection of Cas9 ribonucleoprotein (RNP) complexes in the hippocampus, striatum and cortex. Engineered variants of Cas9 with multiple SV40 nuclear localization sequences enabled a tenfold increase in the efficiency of neuronal editing in vivo. These advances indicate the potential of genome editing in the brain to correct or inactivate the underlying genetic causes of neurological diseases.
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