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PRRT2 inhibits the proliferation of glioma cells by modulating unfolded protein response pathway

Guanghui Bi1, Jingfeng Yan2, Shuzhen Sun3

  • 1Department of Neurology, Dong Ying People's Hospital, Dongying City 257091, Shan dong Province, PR China.

Biochemical and Biophysical Research Communications
|February 14, 2017
PubMed

Insights

Proline-rich transmembrane protein 2 (PRRT2) acts as a tumor suppressor in glioma. Its down-regulation, influenced by microRNA-30a-5p, promotes glioma cell viability and inhibits apoptosis, suggesting PRRT2 as a therapeutic target.

Area of Science:

  • Neuroscience
  • Oncology
  • Molecular Biology

Background:

  • Mutations in the proline-rich transmembrane protein 2 (PRRT2) gene are linked to paroxysmal neurological disorders.
  • The role of PRRT2 in cancer, specifically glioma, remains largely unexplored.

Purpose of the Study:

  • To investigate the function of PRRT2 in glioma tumorigenesis.
  • To elucidate the regulatory mechanisms and therapeutic potential of PRRT2 in glioma.

Main Methods:

  • Large-scale data analysis of PRRT2 expression in glioma tissues versus normal brain tissue.
  • Investigation of PRRT2 regulation by microRNA-30a-5p, mutations, copy number variations, and epigenetic modifications.
  • Cell viability and apoptosis assays following PRRT2 overexpression, with and without microRNA-30a-5p modulation.
  • Analysis of PRRT2's correlation with genes in the unfolded protein response (UPR) pathway.

Main Results:

  • PRRT2 is significantly down-regulated in glioma tissues compared to normal brain tissue.
  • PRRT2 dysregulation is modulated by microRNA-30a-5p, not by mutation, copy number variation, or epigenetic changes.
  • Overexpression of PRRT2 inhibits glioma cell viability and promotes apoptosis, effects reversible by microRNA-30a-5p.
  • PRRT2 expression correlates with genes in the UPR pathway, and its introduction inhibits UPR signaling.

Conclusions:

  • PRRT2 functions as a tumor suppressor in glioma.
  • PRRT2's anti-tumor effects are mediated through the modulation of the UPR pathway.
  • PRRT2 represents a potential therapeutic target for glioma treatment.
Keywords:
GBMPRRT2Unfolded protein responsemicroRNA-30a-5p

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