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Hydrodynamic instability of "stroma-free" hemoglobin
T I Pristoupil1, M Kramlová, T Marík
1Institute of Hematology and Blood Transfusion, Prague, Czechoslovakia.
Biomaterials, Artificial Cells, and Artificial Organs
|January 1, 1989
Summary
Agitation causes fibrous precipitate formation in stroma-free hemolyzates (SFH) due to unstable nonhemoglobin molecules. Further purification is needed for stable, infusable SFH and its variants.
Area of Science:
- Biochemistry
- Hematology
- Materials Science
Background:
- Stroma-free hemolyzates (SFH) are used in various applications.
- The stability and purity of SFH are critical for its use, especially for infusion.
- Uncharacterized precipitates can form in SFH, hindering its application.
Purpose of the Study:
- To investigate the cause of fibrous precipitate formation in agitated SFH.
- To characterize the nature of the fibrous inhomogeneities.
- To identify prerequisites for developing stable, purified SFH for infusion.
Main Methods:
- Kinetic test with visual observation under 4-10x magnification.
- Comparison of agitated SFH with motionless SFH samples.
- Screening experiments to identify fiber-forming substances and precipitation inhibitors.
Main Results:
- Agitation of SFH rapidly induces the formation of fine fibrous inhomogeneities.
- No precipitate formed in SFH samples stored motionless for months.
- Hydrodynamic conditions trigger aggregation of unstable filamentous nonhemoglobin molecules from erythrocyte stromata and other lysed blood cells.
- Numerous attempts failed to significantly remove fiber-forming substances or prevent precipitation.
Conclusions:
- Hydrodynamic conditions are essential for the precipitation of unstable filamentous nonhemoglobin molecules in SFH.
- Existing methods are insufficient to remove these fiber-forming substances.
- Development of hydrodynamically stable and purified SFH is crucial for advancing infusable SFH and its modified variants (MSFH).