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Updated: Mar 7, 2026
11:01
Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Published on: January 18, 2020
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High-Resolution Glycan Analysis by Temperature Gradient Capillary Electrophoresis.
Marton Szigeti1, Andras Guttman1,2
1Horváth Csaba Memorial Institute for Bioanalytical Research, MMKK, University of Debrecen , Debrecen, Nagyerdei krt 98, 4032, Hungary.
Analytical Chemistry
|February 15, 2017
Summary
Temperature gradient capillary electrophoresis improves separation of branched glycans. Optimizing temperature gradients enhances glycan structure resolution, crucial for biotherapeutic analysis.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Separation Science
Background:
- Branched glycans are critical components of biotherapeutics.
- Achieving high separation selectivity for complex glycan structures is challenging.
- Capillary electrophoresis (CE) is a powerful separation technique.
Purpose of the Study:
- To enhance separation selectivities for branched glycans using temperature gradient capillary electrophoresis (TGCE).
- To investigate the impact of temperature on glycan separation.
- To develop an optimized TGCE method for resolving complex oligosaccharide mixtures.
Main Methods:
- Temperature gradient capillary electrophoresis (TGCE) was employed.
- Separations were conducted across a temperature range of 15-45 °C.
- A mixture of afucosylated, fucosylated, and high mannose oligosaccharides was analyzed.
Main Results:
- Separation selectivity was found to be carbohydrate structure dependent.
- Optimal separation temperatures varied among different glycan structures.
- A gradient temperature profile was designed and optimized for complete resolution.
Conclusions:
- Temperature is a critical parameter for manipulating selectivity in glycan separations.
- TGCE offers a powerful approach for analytical glycomics.
- This method enhances the analysis of biotherapeutic-related glycans.
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