MoSe2 Nanolabels for Electrochemical Immunoassays
Rou Jun Toh1, Carmen C Mayorga-Martinez1, Zdeněk Sofer2
1Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University , Singapore 637371.
Analytical Chemistry
|February 15, 2017
Summary
We developed a new method using molybdenum diselenide (MoSe2) nanolabels for detecting biomolecules, crucial for personalized medicine. This technique offers a low-cost, efficient approach to advanced biosensing systems.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Biosensors are increasingly important for personalized medicine.
- Transition-metal dichalcogenides (TMDs) show promise as signal enhancers in biomolecular detection.
Purpose of the Study:
- To develop a solution-based electrochemical exfoliation technique for manufacturing molybdenum diselenide (MoSe2) nanolabels.
- To utilize MoSe2 nanolabels for sensitive and selective protein detection via magneto-immunoassays.
Main Methods:
- Solution-based electrochemical exfoliation using bipolar electrodes to produce MoSe2 nanoparticles (NPs).
- Characterization of MoSe2 NPs for electrocatalytic activity, specifically toward the hydrogen evolution reaction (HER).
- Application of MoSe2 NPs as labels in magneto-immunoassays for protein detection.
Main Results:
- MoSe2 NPs demonstrated significant electrocatalytic activity for HER.
- The developed magneto-immunoassay exhibited excellent selectivity.
- Achieved a wide linear detection range (2-500 ng mL-1) with high sensitivity (Limit of Detection = 1.23 ng mL-1) and good reproducibility (RSD = 9.7%).
Conclusions:
- The electrochemical exfoliation technique is viable and reproducible for producing TMD nanolabels.
- MoSe2 nanolabels are effective for sensitive and selective protein detection in biosensing systems.
- This method facilitates the development of low-cost and efficient biosensing platforms.
