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Updated: Mar 7, 2026

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CCCTC-Binding Factor Transcriptionally Targets Wdr5 to Mediate Somatic Cell Reprogramming.

Feng Wang1,2, Jinghua Han2, Li Wang3

  • 11 Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming, China .

Stem Cells and Development
|February 15, 2017
PubMed
Summary

CTCF regulates WD repeat domain 5 (Wdr5) expression and function. CTCF knockdown impairs induced pluripotent stem cell formation, but Wdr5 overexpression can rescue these defects, highlighting their crucial roles in pluripotency.

Keywords:
CTCFWdr5pluripotencyreprogrammingstem cells

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Area of Science:

  • Epigenetics and developmental biology
  • Stem cell biology
  • Gene regulation

Background:

  • WD repeat domain 5 (Wdr5) is essential for maintaining embryonic stem cell (ES) pluripotency and somatic cell reprogramming.
  • The precise regulatory network upstream of Wdr5 in these processes is not fully understood.

Purpose of the Study:

  • To investigate the role of CTCF as an upstream regulator of Wdr5.
  • To elucidate the molecular mechanism by which CTCF controls Wdr5 expression and function.
  • To determine the functional consequence of the CTCF-Wdr5 interaction in cellular reprogramming.

Main Methods:

  • Co-immunoprecipitation to assess physical association between CTCF and Wdr5.
  • Chromatin immunoprecipitation followed by sequencing (ChIP-seq) to identify Wdr5 binding sites.
  • Quantitative real-time PCR (qRT-PCR) and Western blotting to measure gene and protein expression.
  • CRISPR-Cas9 mediated knockdown of CTCF and subsequent rescue experiments with Wdr5 overexpression.

Main Results:

  • CTCF physically interacts with Wdr5.
  • CTCF directly binds to the Wdr5 gene promoter, regulating its transcriptional expression.
  • Knockdown of CTCF leads to impaired ES cell pluripotency and reduced induced pluripotent stem cell formation.
  • Overexpression of Wdr5 rescues these cellular defects caused by CTCF knockdown.

Conclusions:

  • CTCF acts as a critical upstream regulator of Wdr5.
  • The physical and transcriptional regulation of Wdr5 by CTCF is essential for maintaining pluripotency and successful somatic cell reprogramming.
  • CTCF and Wdr5 function in a coordinated manner to re-establish pluripotency.