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Sequential morphological changes in the pig detrusor in response to chronic partial urethral obstruction
J S Dixon1, C J Gilpin, S A Gilpin
1Department of Cell and Structural Biology, University of Manchester.
British Journal of Urology
|October 1, 1989
Summary
Partial urinary bladder outflow obstruction in pigs initially reduces autonomic nerves. Over time, connective tissue infiltrates detrusor muscle, but muscle cells don't significantly enlarge, suggesting complex bladder wall adaptations.
Area of Science:
- Urology
- Pathology
- Morphology
Background:
- Partial outflow obstruction is a common cause of bladder dysfunction.
- Understanding the structural adaptations of the bladder wall is crucial for managing bladder diseases.
Purpose of the Study:
- To investigate the sequential morphological changes in the porcine urinary bladder wall following experimentally induced partial outflow obstruction.
- To characterize alterations in nerve density, smooth muscle morphology, and connective tissue distribution.
Main Methods:
- Morphometric analysis using light and electron microscopy.
- Experimental induction of partial urethral obstruction in pigs.
- Evaluation of bladder wall components over varying obstruction durations.
Main Results:
- A 50% reduction in autonomic nerve density within the first 3 months of obstruction.
- Gradual further decrease in innervation with longer obstruction periods.
- Connective tissue infiltration of detrusor muscle bundles starting from 3 months onwards.
- No significant change in smooth muscle cell size detected after 12 months of obstruction.
- Smooth muscle cells showed increased perinuclear granular reticulum, especially with prolonged obstruction.
Conclusions:
- Partial outflow obstruction induces significant early changes in bladder innervation.
- Progressive connective tissue remodeling occurs in the bladder wall over time.
- The observed cellular changes, including increased granular reticulum, suggest adaptive responses to obstruction, though underlying mechanisms require further investigation.