[Immunoscintigraphy in experiments and clinical practice: its possibilities, advantages and limitations]

Area of Science:

• Nuclear medicine
• Oncology
• Immunology

Background:

• Antibody-based imaging offers targeted tumor detection.
• Radionuclide labeling enhances antibody visibility for diagnostic purposes.

Purpose of the Study:

• To describe the process of preparing, isolating, and labeling antibodies with radionuclides.
• To evaluate the efficacy of labeled antibodies and their fragments in immunoscintigraphy for tumor imaging.
• To present clinical results using commercial antibody kits for digestive tract tumors.

Main Methods:

• Antibody and F(ab)2 fragment preparation and radionuclide labeling.
• In vivo biodistribution studies with labeled antibodies and fragments.
• Immunoscintigraphic imaging of tumors in preclinical and clinical settings.
• Utilizing commercial kits like Iodomab and Imacis for patient studies.

Main Results:

• Successful preparation and labeling of antibodies and F(ab)2 fragments.
• Demonstrated tumor uptake and favorable biodistribution patterns in experimental studies.
• Clinical immunoscintigraphy revealed diagnostic potential for digestive tract tumors using commercial kits.

Conclusions:

• Antibody-based immunoscintigraphy is a viable technique for tumor detection.
• Optimized antibody labeling and fragment selection are crucial for effective imaging.
• Commercial kits show promise for clinical application in diagnosing gastrointestinal cancers.