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Plasma prefractionation methods for proteomic analysis and perspectives in clinical applications
Somchai Chutipongtanate1, Supawat Chatchen2, Jisnuson Svasti3,4
1Pediatric Translational Research Unit, Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Salaya, Thailand.
Proteomics. Clinical Applications
|February 15, 2017
Summary
Plasma prefractionation enhances biomarker discovery by reducing protein complexity. This review covers methods and clinical applications for detecting low-abundance proteins in proteomic analysis.
Area of Science:
- Proteomics
- Clinical Chemistry
- Biomarker Discovery
Background:
- Plasma contains numerous clinically relevant biomarkers.
- High protein concentration variability challenges the detection of low-abundance proteins.
- Plasma prefractionation is crucial for simplifying samples and enabling deeper proteomic analysis.
Purpose of the Study:
- To review common plasma prefractionation techniques for proteomic analysis.
- To discuss considerations for applying these methods in clinical settings.
- To highlight examples of plasma prefractionation in clinical applications.
Main Methods:
- Review of established plasma prefractionation methodologies.
- Analysis of challenges and solutions for clinical proteomic workflows.
- Case study review of prefractionation in clinical biomarker research.
Main Results:
- Various prefractionation methods effectively reduce plasma complexity.
- Successful application of prefractionation enables detection of low-abundance biomarkers.
- Clinical implementation requires careful consideration of method robustness and scalability.
Conclusions:
- Plasma prefractionation is essential for advancing clinical proteomics.
- Effective prefractionation strategies improve biomarker identification and validation.
- Further development is needed to optimize methods for routine clinical use.
