Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitosis and Cytokinesis01:35

Mitosis and Cytokinesis

11.9K
In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
11.9K
Mitosis and Cytokinesis02:03

Mitosis and Cytokinesis

283.4K
In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
283.4K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

6.7K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.7K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

3.5K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.5K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.9K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TREX2 component PCID2 scaffolds alternative SAC3-based subcomplexes with distinct RNA processing and export function.

bioRxiv : the preprint server for biology·2026
Same author

The γ-Tubulin Ring Complex promotes mitotic spindle integrity and acts as a microtubule minus-end cap during mitosis.

bioRxiv : the preprint server for biology·2026
Same author

Interphase chromosome conformation is specified by distinct folding programmes inherited through mitotic chromosomes or the cytoplasm.

Nature cell biology·2025
Same author

A genome-wide RNAi screen for novel CIN genes using human artificial chromosome.

PNAS nexus·2025
Same author

Lamin A/C loss promotes R-loop-mediated genomic instability and poor survival in small-cell lung cancer.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Lamin A/C Deficiency Drives Genomic Instability and Poor Survival in Small-Cell Lung Cancer through Increased R-loop Accumulation.

bioRxiv : the preprint server for biology·2025
Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026
Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026
Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026
Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

9.0K

The SUMO Pathway in Mitosis.

Debaditya Mukhopadhyay1, Mary Dasso2

  • 1Section on Cell Cycle Regulation, Laboratory of Gene Regulation and Development, National Institute of Child Health and Development, National Institutes of Health, 18 Library Drive, Room 106, Building 18T, Bethesda, MD, 20892, USA.

Advances in Experimental Medicine and Biology
|February 16, 2017
PubMed
Summary
This summary is machine-generated.

Sumoylation, a protein modification, is crucial for accurate cell division (mitosis). This review explores how sumoylation regulates key mitotic events like chromosome condensation and cytokinesis, ensuring genetic material is equally distributed.

Keywords:
CondensinKinetochoreMitosisSUMOTopoisomerase

More Related Videos

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

12.7K
SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.7K

Related Experiment Videos

Last Updated: Mar 7, 2026

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

9.0K
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

12.7K
SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.7K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Mitosis requires precise chromosome segregation for daughter cell genetic equality.
  • Post-translational modifications, including phosphorylation and ubiquitination, are vital for mitotic regulation.
  • Sumoylation, a conjugation to small ubiquitin-like modifiers (SUMO), is increasingly recognized for its role in mitosis.

Purpose of the Study:

  • To review the essential regulatory roles of sumoylation during mitosis.
  • To discuss the fundamental biology of the sumoylation pathway.
  • To explore the involvement of SUMOylation in specific mitotic functions and identify potential protein targets.

Main Methods:

  • Literature review of existing research on sumoylation and mitosis.
  • Analysis of studies implicating SUMOylation in chromosome condensation, centromere/kinetochore organization, and cytokinesis.
  • Discussion of identified cellular protein targets of sumoylation during mitosis.

Main Results:

  • Sumoylation plays critical roles in organizing chromosomes during mitosis.
  • The SUMO pathway is involved in centromere/kinetochore function and the completion of cell division (cytokinesis).
  • Specific cellular proteins targeted by sumoylation are being identified as key regulators of these mitotic processes.

Conclusions:

  • Sumoylation is an essential post-translational modification regulating multiple aspects of mitosis.
  • Understanding SUMOylation's targets and mechanisms provides insight into ensuring accurate cell division.
  • Further research into sumoylation pathways can reveal novel therapeutic targets for mitotic dysfunction.