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Viral Nanoparticles for In vivo Tumor Imaging

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Published on: November 16, 2012

Engineering Recombinant Virus-like Nanoparticles from Plants for Cellular Delivery.

Lou Brillault, Philippe V Jutras, Noor Dashti

¹Department of Biological Chemistry, John Innes Centre , Norwich Research Park, Colney, Norfolk NR4 7UH, United Kingdom.

|February 16, 2017

Summary

Researchers engineered Bluetongue virus (BTV) core-like particles (CLPs) using plant expression systems. These engineered viral nanoparticles demonstrate potential for targeted drug delivery by binding to cell surface receptors.

Keywords:

bioconjugation bluetongue virus core-like particle cryo-EM guest protein encapsidation receptor targeting virus-like nanoparticle

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Area of Science:

Biophysics
Nanotechnology
Molecular Biology

Background:

Virus-like nanoparticles (VNPs) are crucial for biomedical and nanotechnology applications.
Understanding viral capsid assembly is key to designing and modifying VNPs.
Bluetongue virus (BTV) structural proteins offer potential for VNP development.

Purpose of the Study:

To investigate the plant-based transient expression of BTV structural proteins VP3 and VP7.
To characterize the structure and assembly of recombinant BTV core-like particles (CLPs).
To evaluate the potential of BTV CLPs for targeted cargo delivery.

Main Methods:

Plant-based transient expression of BTV VP3 and VP7 proteins.
Production of empty and green fluorescent protein (GFP)-encapsidating CLPs.
Single-particle cryo-electron microscopy for structural analysis.
Fluorescent labeling of CLPs and cell binding assays.

Main Results:

High yields of empty and GFP-encapsidating CLPs were obtained from plant leaves.
Recombinant CLPs exhibited identical external structures but differed internally from infection-derived CLPs.
Labeled CLPs successfully bound human integrin via an Arg-Gly-Asp motif.
Fluorescently labeled CLPs interacted with cells overexpressing surface receptors.

Conclusions:

BTV CLPs produced via plant expression are structurally distinct yet externally consistent.
The engineered BTV CLPs can be internally labeled and target specific cell surface receptors.
BTV CLPs show promise as versatile tools for targeted cargo delivery applications.
Detailed structural analysis is vital for validating and optimizing VNP design.