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Carbonic anhydrase I and II autoantibodies in Behçet's disease
Ahmet Mentese1, Ahmet Alver1, Selim Demir2
1Faculty of Medicine, Karadeniz Technical University.
Insights
Carbonic anhydrase (CA) autoantibodies were significantly elevated in Behçet
Area of Science:
- Immunology
- Rheumatology
- Biochemistry
Background:
- Behçet's disease is a systemic vasculitis characterized by oral and genital ulcerations, skin lesions, and uveitis.
- Carbonic anhydrase (CA) is a crucial metalloenzyme involved in acid-base balance.
- Anti-CA antibodies are implicated in various autoimmune disorders.
Purpose of the Study:
- To investigate the presence and levels of carbonic anhydrase I (CA I) and carbonic anhydrase II (CA II) autoantibodies in patients with Behçet's disease (BD).
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was employed to measure CA I and CA II autoantibody levels.
- The study included 35 patients diagnosed with Behçet's disease and 29 healthy controls.
Main Results:
- Patients with Behçet's disease exhibited significantly higher levels of both CA I and CA II autoantibodies compared to healthy controls (p=0.013 and p<0.0001, respectively).
- Specific cut-off values for anti-CA I (0.250 ABSU) and anti-CA II (0.171 ABSU) demonstrated high diagnostic potential, with 100% specificity for predicting BD.
Conclusions:
- Elevated CA I and CA II autoantibody levels are associated with Behçet's disease.
- These findings suggest a potential role for carbonic anhydrase autoantibodies in the pathogenesis of Behçet's disease.
Background:
Behçet's disease is a vasculitis, seen more frequently around the Mediterranean and the Far East, and evinces with oral and genital ulcerations, skin lesions and uveitis. Carbonic anhydrase (CA) is a metalloenzyme which is widely distributed in the living world, and it is essential for the regulation of acid-base balance. Anti-CA antibodies have been reported in many disorders, such as systemic lupus erythematosus, Sjögren's syndrome, rheumatoid arthritis, endometriosis, idiopathic chronic pancreatitis, type 1 diabetes and Graves' disease. The goal of this study was to investigate CA I and II autoantibodies in Behçet's disease (BD).
Methods:
35 patients with BD and 29 healthy controls were included in the study and CA I and II autoantibody levels were investigated by ELISA.
Results:
The CA I and II autoantibody levels of BD group were significantly higher than the healthy group (p=0.013, p inf 0.0001, respectively). A cut-off value of 0.250 ABSU for anti-CA I was associated with 34 % sensitivity and 100 % specificity and a cut-off value of 0.171 ABSU for anti-CA II was associated with 54 % sensitivity and 100 % specificity for predicting BD.
Conclusion:
The CA I and II autoantibody levels in patients with BD were found higher compared to control group and the results suggest that CA I and II autoantibodies may be involved in the pathogenesis of BD.
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