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Proteomic Profiling Analysis Reveals a Link between Experimental Autoimmune Uveitis and Complement Activation in

D D Guo1, B Hu2, H Y Tang3

  • 1Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, China.

Scandinavian Journal of Immunology
|February 16, 2017
PubMed
Summary

This study reveals that experimental autoimmune uveitis (EAU) in rats involves significant changes in plasma proteins, particularly those linked to complement and coagulation cascades, offering new insights into uveitis pathogenesis.

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Area of Science:

  • Ophthalmology
  • Immunology
  • Proteomics

Background:

  • Uveitis is an autoimmune ocular disease impacting vision.
  • Its precise pathogenic mechanisms involving protein expression and signaling pathways require further elucidation.

Purpose of the Study:

  • To investigate the proteomic changes in experimental autoimmune uveitis (EAU) in rats.
  • To identify key proteins and pathways involved in uveitis pathogenesis.

Main Methods:

  • Induced EAU in rats.
  • Quantified C3a and soluble C5b-9 (sC5b-9) in plasma and aqueous humor.
  • Utilized liquid chromatography-tandem mass spectrometry and bioinformatics for plasma protein analysis.

Main Results:

  • Identified 168 differentially expressed plasma proteins in EAU rats.
  • Observed elevated levels of sC5b-9 and C3a in EAU rat plasma and aqueous humor.
  • Bioinformatic analysis linked differentially expressed proteins to complement and coagulation cascades, immune, and metabolic processes.

Conclusions:

  • Complement and coagulation cascades are significantly implicated in uveitis pathogenesis.
  • Aberrant protein expression in plasma is a key feature of EAU.
  • Findings provide a deeper understanding of uveitis mechanisms, potentially aiding therapeutic strategies.