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Related Experiment Video

Updated: Mar 7, 2026

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

Advances in multicellular spheroids formation.

X Cui¹, Y Hartanto¹, H Zhang²

¹School of Chemical Engineering, University of Adelaide, Adelaide, South Australia 5005, Australia.

Journal of the Royal Society, Interface

|February 17, 2017

Summary

This summary is machine-generated.

Three-dimensional multicellular spheroids (MCSs) mimic in vivo environments for tissue engineering. Microfluidic hydrogel methods offer promising fabrication for cell-cell and cell-matrix interactions.

Keywords:

hydrogel microfluidics multicellular spheroids three-dimensional culture tissue engineering

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Last Updated: Mar 7, 2026

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Area of Science:

Biomaterials Science
Tissue Engineering
Cell Biology

Background:

Three-dimensional multicellular spheroids (MCSs) replicate complex in vivo microenvironments.
MCSs are crucial for bridging the gap between in vitro and in vivo research models.
They exhibit intricate architectural structures and dynamic cell-cell/cell-matrix interactions.

Purpose of the Study:

To review the structure and biology of multicellular spheroids (MCSs).
To detail various methods for fabricating MCSs.
To highlight promising microfluidic approaches for MCS formation.

Main Methods:

Review of existing literature on MCS structure, biology, and fabrication.
Focus on microfluidic techniques utilizing hydrogel support for MCS generation.
Analysis of methods enabling controlled cell-cell and cell-matrix interactions.

Main Results:

Multicellular spheroids (MCSs) offer a bio-mimicking in vivo microenvironment.
Various fabrication techniques for MCSs are discussed.
Microfluidics with hydrogel support shows significant promise for MCS formation.

Conclusions:

Multicellular spheroids (MCSs) are fundamental building blocks for tissue reconstruction.
Microfluidic hydrogel-based fabrication enables essential cell interactions in confined spaces.
This approach advances the development of more accurate in vitro models.